AMB Volume 42, Issue 2, 2026 Pages 351-360 https://doi.org/10.59393/amb26420217
Production and Partial Purification of a Bacteriocin Produced by Virgibacillus salarius POTR191
Berberov K., Atanasova N., Engibarov S., Lazarkevich I., Yakimova B., Paunova T., Boyadzhieva I., Kabaivanova L.
The urgent need for novel antibiotics requires discovery and characterization of novel antibacterial compounds. Halophilic bacteria produce various secondary metabolites with superior properties and antibacterial compounds are of particular interest. Here, we described the production of a bacteriocin from the halophilic bacterium Virgibacillus salarius POTR191. The fermentation conditions were varied to achieve maximal yield. Then, the bacteriocin was partially purified by ammonium sulfate precipitation and ethyl acetate extraction. The purity was checked by Tricine-SDS-PAGE. The optimized fermentation conditions and extraction method led to 36% yield increase. The bacteriocin with an approximate molecular mass of 2.2 kDa was maximally produced after 36 h of batch cultivation. The proposed three-step partial purification scheme led to a 73.77-fold purification fold and a yield of 6.4% with a specific activity of the bacteriocin extract of 1951.22 AU/mg.
Keywords: bacteriocin, halophilic bacteria, optimization, purification, saltern, Virgibacillus
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